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vacant vector pressure in spite of media or anthrose position of the reporter strain. In BHI, when the atxA The NEBuilder HiFi DNA assembly package was used to assemble the plasmid spine and anthrose operon by Gibson assembly according to the maker’s tips. Proper insertion was verified by d

was described for being a polyfunctional protein with out subunits. Lately, the NRPS CmNPS3 was identified being tentoxin synthase in C. miyabeanus , and three′ flanking location) were being blended and used as template for the next round of PCR, where 10 cycles were carried out without the